Detection of the <i>JAK2</i>V617F Mutation in Myeloproliferative Disorders by Melting Curve Analysis Using the LightCycler System

Abstract

These findings demonstrate the suitability of this assay for identifying JAK2(V617F) in a clinical laboratory setting. Furthermore, the semiquantitative detection of JAK2(V617F) in archived specimens provides a new tool for studying the prognostic significance of this mutation.