Selective and sensitive detection and quantification of arylamine N-acetyltransferase 2 by a ratiometric fluorescence probe

Abstract

A novel NAT2 specific probe has been developed for sensitive detection of the activity of NAT2. Upon NAT2 catalyzed acetylation of the molecule to N-acetyl-amonafide, the ratiometric response of fluorescence with significant changes in wavelength and intensity is observed. Its significant application potential has been successfully demonstrated in cellular systems.