Growth of animal cells on microbeads. I. <i>In situ</i> estimation of numbers

Abstract

Evaluation of the number of anchorage-dependent cells growing on the surface of microbeads kept in suspension in a bioreactor is a tedious procedure fraught with many difficulties. Using the Coulter counter as a biomass probe, this article shows that the number of cells adhering to microbeads can be determined while the cells are still attached.