Predominant cultivable microbiota in periodontosis

TLDR

The study collected anaerobic samples from the apical portion of periodontosis lesions and from clinically normal sites in 5 patients using a specialized device, then isolated and differentiated Gram‑negative anaerobic rods into five groups based on morphology, culture, and biochemistry, and confirmed similar groups in an additional 9 patients. Pocket sites in periodontosis lesions were dominated by Gram‑negative anaerobic rods, contrasting with the predominantly Gram‑positive flora of control sites, and similar rod groups were identified in an additional nine patients.

Abstract

The predominant cultivable microbiota of periodontosis lesions was studied in 5 patients using a specially designed anaerobic sampling device and current anaerobic methodology. Two samples from the apical portion of periodontosis lesions and one sample from clinically normal sites were taken from each patient. The results indicated that samples obtained from pocket sites consisted of significantly increased proportions of Gram negative anaerobic rods when compared to a predominantly Gram positive flora in the control sites. Many of these organisms could not be classified. An additional 9 periodontosis patients were sampled and it was determined that groups of Gram negative anaerobic rods from similar to the first 5 patients were isolated. These organisms were differentiated into 5 groups based on morphologic, cultural, and biochemical characteristics.