Potential of Different Enzyme Immobilization Strategies to Improve Enzyme Performance

TLDR

Enzyme biocatalysis is vital for industries such as energy, food, and fine chemistry, and immobilization—on supports, support‑free aggregates, or nanoparticles—enables enzyme reuse while improving purity, stability, activity, specificity, and selectivity. The paper reviews the pros and cons of various immobilization strategies and offers guidance on selecting the optimal approach for specific enzymes and processes. The authors compare non‑porous supports with conventional porous ones and outline criteria—substrate nature and company requirements such as volumetric activity and stability—to guide the choice of immobilization strategy.

Abstract

Abstract Enzyme biocatalysis plays a very relevant role in the development of many chemical industries, e.g., energy, food or fine chemistry. To achieve this goal, enzyme immobilization is a usual pre‐requisite as a solution to get reusable biocatalysts and thus decrease the price of this relatively expensive compound. However, a proper immobilization technique may permit far more than to get a reusable enzyme; it may be used to improve enzyme performance by improving some enzyme limitations: enzyme purity, stability (including the possibility of enzyme reactivation), activity, specificity, selectivity, or inhibitions. Among the diverse immobilization techniques, the use of pre‐existing supports to immobilize enzymes (via covalent or physical coupling) and the immobilization without supports [enzyme crosslinked aggregates (CLEAs) or crystals (CLECs)] are the most used or promising ones. This paper intends to give the advantages and disadvantages of the different existing immobilization strategies to solve the different aforementioned enzyme limitations. Moreover, the use of nanoparticles as immobilization supports is achieving an increasing importance, as the nanoparticles versatility increases and becomes more accessible to the researchers. We will also discuss here some of the advantages and drawbacks of these non porous supports compared to conventional porous supports. Although there are no universal optimal solutions for all cases, we will try to give some advice to select the optimal strategy for each particular enzyme and process, considering the enzyme properties, nature of the process and of the substrate. In some occasions the selection will be compulsory, for example due to the nature of the substrate. In other cases the optimal biocatalyst may depend on the company requirements (e.g., volumetric activity, enzyme stability, etc).