Mapping the Transcription Start Points of the<i>Staphylococcus aureus eap</i>,<i>emp</i>, and<i>vwb</i>Promoters Reveals a Conserved Octanucleotide Sequence That Is Essential for Expression of These Genes

Abstract

Mapping the transcription start points of the eap, emp, and vwb promoters revealed a conserved octanucleotide sequence (COS). Deleting this sequence abolished the expression of eap, emp, and vwb. However, electrophoretic mobility shift assays gave no evidence that this sequence was a binding site for SarA or SaeR, known regulators of eap and emp.