Daily short exposure of cultured mesothelial cells to lactated, high-glucose, low-pH peritoneal dialysis fluid induces a low-profile regenerative steady state

Abstract

This study was designed to evaluate cytotoxic effects and influence upon cell growth of cultured mesothelial cells exposed to modified 4.25% Dianeal dialysate fluid (M-199 in Dianeal solution, glucose 4.25 g, pH 5.2) (Expr. group), 60 min a day for a total period of follow-up of 13 consecutive days, compared with that observed in a control group (C). Beginning on day 7, the cell counts in group C were significantly higher than those observed at zero time (P < 0.05). Cell counts in the experimental group showed no significant differences between the first day of culture and each one of the 13 consecutive days of follow-up. Thymidine incorporation into DNA observed on the first day in C, was significantly higher (P < 0.01) beginning on the 10th day. Values observed in the experimental group were low during the whole period of follow-up. LDH mean values at each time interval, were significantly higher (P < 0.01 and < 0.001) for cells exposed to the dialysis solution. Repeated exposure of the mesothelium to 40 mMol/l lactate and high glucose concentrations induced severe cell injury and death, decreased cell growth and, consequently, a reduced rate of regeneration which is extended as long as the repeated exposure is maintained.