POST OPERATIVE WOUND INFECTION: BACTERIOLOGY AND ANTIBIOTIC SENSITIVITY PATTERN

Abstract

Background: Wound infection occurs if the integrity and protective function of the skin is breached. Most Bacteria, certain Viruses (e. g. Herpes virus), Fungi (e.g. Candida albicans) are responsible for wound infection [3]. A study was designed to isolate and identify the aerobic bacterial agents of post operative wound infection as well as to determine their antibiotic sensitivity pattern. Method: Total 102 pus samples were collected from surgical sites and immediately inoculated on Blood agar and MacConkey agar plates. Then the culture plates were incubated at 37oC for 24 hours. After incubation, all isolates were identified by using Gram stain and biochemical methods. Sensitivity tests were performed on Mueller Hinton agar plate by Kirby Bauer’s disc diffusion technique. Result: During the study period (February 2012- January 2013), a total of 102 samples were analyzed. Among 102 samples, 73 (71.5%) showed positive growth. The most frequent isolate was Staphylococcus aureus 24 (32.8%) followed by Escherichia coli 15 (20.5%), Pseudomonas species (16.4%). Antibiotic sensitivity test of the isolates showed that Ampicillin+Sulbactum (87.5%) and Linazolid (85%) were the most effective antibiotics for Gram positive bacteria and Ciprofloxacin (52.5%) was the least effective antibiotic. Gram negative isolates were most sensitive to Lomifloxacin (70.3%) followed by Netilline (61.1%). Cefuroxime (18.5%) was the least sensitive antibiotic for Gram negative bacteria in this study. Conclusion: The most common isolate in wound infection was Staphylococcus aureus followed by E.coli. Ampicillin+Sulbactum was most effective antibiotic for Gram positive bacteria and Lomifloxacin was most effective against Gram negative bacteria.