Human Molecular Genetics · 1993 · 68 citations · 0 references
Life SciencesGeneticsMolecular GeneticsCancer GeneticsMolecular OncologyNeoplasiaCancer CytogeneticsLoss Of HeterozygosityMolecular CytogeneticsTumor Suppressor Gene InactivationTumor Suppressor GenesChromosome 9P LossGenomic Deletion DetectionLoss Of Heterozygosity MappingRestriction Fragment Length Polymorphism AnalysisTumor Suppressor Gene AlterationsTumor Suppressor Gene AnalysisTumor Suppressor Gene DeletionsTumor Suppressor Gene Loss
A panel of 18 superficial or invasive transitional cell carcinomas (TCCs) was analyzed for chromosome 9 deletions by performing a high-density loss of heterozygosity (LOH) analysis. Twenty-five microsatellite loci were assayed by the polymerase chain reaction (PCR) and 7 restriction fragment length polymorphism (RFLP) loci were analyzed by Southern blotting. Concordant results were obtained with these methods, including direct comparisons at 2 loci. Chromosome 9 LOH was observed in 13 (72%) of 18 informative cases, including 10 superficial lesions. In contrast, LOH on chromosomes 10, 15, 20 and 21 was < or = 8%. Evidence for missing copies of chromosome 9 was observed in 7 of 13 LOH cases. Comparison of cases with subchromosomal LOH implicated the region between the D9S55 locus on 9p12 and the argininosuccinate synthetase (ASS) locus on 9q34.1 as the location of a tumor suppressor gene relevant to TCC.