Identification of <i>Staphylococcus</i> spp. by PCR-Restriction Fragment Length Polymorphism of <i>gap</i> Gene

Abstract

Oligonucleotide primers specific for the Staphylococcus aureus gap gene were previously designed to identify 12 Staphylococcus spp. by PCR. In the present study, AluI digestion of PCR-generated products rendered distinctive restriction fragment length polymorphism patterns that allowed 24 Staphylococcus spp. to be identified with high specificity.