Quantification of Protein Sulfenic Acid Modifications Using Isotope‐Coded Dimedone and Iododimedone

Abstract

Quantitative proteomics: The new technique mentioned in the title—in short, ICDID—enables quantification of sulfenic acid modifications in proteins (see picture). The approach permits S-hydroxylation site occupancy to be monitored at individual cysteines within a single protein and is compatible with peptide-based proteomic strategies.