Cryopreservation and Enumeration of Human Endothelial Progenitor and Endothelial Cells for Clinical Trials

Abstract

Our data indicate that cryopreservation at -80°C for 7 days decreases, although not significantly, viability of PBMC and numbers of subsets of EC and EPC. This method may provide an optimized approach to isolation and short-term cryopreservation of subsets of EPC and of mature EC suitable for multicenter trials.