No sex‐specific markers detected in bluegill sunfish <i>Lepomis macrochirus</i> by AFLP

Abstract

The amplified fragment-length polymorphism (AFLP) technique was used to identify sex-specific markers in bluegill sunfish. A total of 12 835 loci were produced by 256 primer combinations, of which nine (0.73 per thousand) exhibited presumed sex-associated amplifications in the pooled samples; however, none of which revealed sex specificity upon individual evaluation.