Segmental isotope labeling of proteins for NMR structural study using a protein S tag for higher expression and solubility

NMRPipe: A multidimensional spectral processing system based on UNIX pipes

Frank Delaglio, Stephan Grzesiek, Geerten W. Vuister et al. · Journal of Biomolecular NMR · 1995 · 16.3K citations

Computing, Computer Science, Computer Systems +14

Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase

Donald B. Smith, Kevin S. Johnson · Gene · 1988 · 6K citations

Life Sciences, Biochemistry, Molecular Biology +12

TALOS+: a hybrid method for predicting protein backbone torsion angles from NMR chemical shifts

Yang Shen, Frank Delaglio, Gabriel Cornilescu et al. · Journal of Biomolecular NMR · 2009 · 2.6K citations

Life Sciences, Biochemistry, Bioinformatics +11

Consistent blind protein structure generation from NMR chemical shift data

Yang Shen, Oliver F. Lange, Frank Delaglio et al. · Proceedings of the National Academy of Sciences · 2008 · 864 citations · Full text

Life Sciences, Biochemistry, Chemistry +16

Vectors that facilitate the expression and purification of foreign peptides in Escherichia coli by fusion to maltose-binding protein

Chu di Guana, Ping Lib, Paul D. Riggsa et al. · Gene · 1988 · 675 citations

Life Sciences, Biotechnology, Molecular Biology +9