American Journal of Tropical Medicine and Hygiene · 2005 · 59 citations · 27 references
Veterinary MedicineImmunologyInfectious DiseasesDiagnostic ParasitologyImmunology Of Infectious DiseasesImmunopathologyAntigen Detection AssaysDiagnostic ImmunologyHelminth DiagnosticsPathogen DetectionSerological DiagnosisSerological DiagnosticsCyst AntigensDiagnostic AntigensImmunodiagnostic BiomarkersParasitic Antigen DetectionSerological AntigensSerological Test ValidationTaenia Solium DetectionWestern Blot Analysis
The gold standard serodiagnostic assay for cysticercosis and neurocysticercosis, diseases caused by the metacestode of Taenia solium, uses lentil lectin-purified glycoprotein (LLGP) in a Western blot assay. We tested two antigens derived from LLGP, synthetic TS18var1 (sTS18var1) and recombinant GP50 antigen (rGP50), in an enzyme-linked immunosorbent assay (ELISA) using serum and cerebrospinal fluid (CSF) samples. The sensitivity for serum and CSF was 94.7% and 100% for rGP50 and 90.4% and 90.2% for sTS18var1, respectively. The specificity for serum and CSF samples was 93.8% and 100% for rGP50 and 90.3% and 98.0% for sTS18var1, respectively. The use of these antigens individually or combined as a diagnostic antigen cocktail eliminates the need for purification of antigens from parasite material and offers the advantage of using a simple and quantitative ELISA format.
27
Index for rating diagnostic tests
W. J. Youden · Cancer · 1950 · 11.5K citations · Full text
Education, Educational Assessment, Educational Measurement +11
A proposal to declare neurocysticercosis an international reportable disease.
Gustavo C. Román, Julio Sotelo, Oscar Del Brutto et al. · PubMed · 2000 · 287 citations