Molecular Detection of <i>Histoplasma capsulatum</i> var. <i>capsulatum</i> in Human Clinical Samples

Abstract

We developed a seminested PCR for the diagnosis of histoplasmosis that amplifies a portion of the Histoplasma capsulatum H antigen gene. This assay is highly sensitive and specific, being able to detect genomic material corresponding to less than 10 yeast cells without cross-reaction against other bacterial or fungal pathogens.