Sidedness of plant plasma membrane vesicles purified by partitioning in aqueous two‐phase systems

Abstract

Preparations of plant plasma membrane vesicles were obtained by partition in dextran‐polyethylene glycol two‐phase systems. By this procedure particles are separated according to their surface properties, and an iso‐osmotic environment is maintained throughout. The vesicles thus produced are right‐side‐out and sealed, as measured by enzyme [(K + + Mg 2+ )‐ATPase and glucan synthetase II] latency on addition of Triton X‐100.