Ecology · 1998 · 332 citations · 105 references
Life SciencesBiologyMolecular BiologyConservation GeneticsGenomicsSpecies ConservationMetapopulation ConservationPolymerase Chain ReactionMetapopulation Genetic StructureRandom Amplified Polymorphic Dna AnalysisRandom Amplified Polymorphic Dna MarkersRibosomal Dna AnalysisSpecies Conservation Prioritization
Recent advances in molecular technology have opened a new chapter in species conservation efforts, as well as population biology. DNA sequencing, MHC (major histocompatibility complex), minisatellite, microsatellite, and RAPD (random amplified polymorphic DNA) procedures allow for identification of parentage, more distant relatives, founders to new populations, unidentified individuals, population structure, effective population size, population-specific markers, etc. PCR (polymerase chain reaction) amplification of mitochondrial DNA, nuclear DNA, ribosomal DNA, chloroplast DNA, and other systems provide for more sophisticated analyses of metapopulation structure, hybridization events, and delineation of species, subspecies, and races, all of which aid in setting species recovery priorities. Each technique can be powerful in its own right but is most credible when used in conjunction with other molecular techniques and, most importantly, with ecological and demographic data collected from the field. Surprisingly few taxa of concern have been assayed with any molecular technique. Thus, rather than showcasing exhaustive details from a few well-known examples, this paper attempts to present a broad range of cases in which molecular techniques have been used to provide insight into conservation efforts.
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Genetics and Demography in Biological Conservation
Russell Lande · Science · 1988 · 2.5K citations
Microsatellite analysis of population structure in Canadian polar bears
David Paetkau, Wendy Calvert, I Stirling et al. · Molecular Ecology · 1995 · 2.1K citations
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