Concepedia

Rapid screening of antibody–antigen binding using dynamic light scattering (DLS) and gold nanoparticles

Yen H. Lai, Sunghyu Koo, Soon Hwan Oh, Elizabeth A. Driskell, Jeremy D. Driskell

Analytical Methods · 2015 · 57 citations · 20 references

DOI

Concepts

Abstract

Antigen-mediated aggregation of antibody–gold nanoparticle conjugates is detected with dynamic light scattering to evaluate antibody–antigen binding.

References

20

A simple method of estimating 50 per cent end points

Hr Muench · Medical Entomology and Zoology · 1938 · 8.4K citations

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Enzyme immunoassays in diagnostic medicine. Theory and practice.

A. Voller, D. E. Bidwell, Ann Bartlett · PubMed · 1976 · 1.1K citations

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A One-Step Homogeneous Immunoassay for Cancer Biomarker Detection Using Gold Nanoparticle Probes Coupled with Dynamic Light Scattering

Xiong Liu, Qiu Dai, Lauren A. Austin et al. · Journal of the American Chemical Society · 2008 · 669 citations

Adsorption of horseradish peroxidase, ovomucoid and anti-immunoglobulin to colloidal gold for the indirect detection of concanavalin A, wheat germ agglutinin and goat anti-human immunoglobulin G on cell surfaces at the electron microscopic level: a new method, theory and application.

William D. Geoghegan, G. Adolph Ackerman · Journal of Histochemistry & Cytochemistry · 1977 · 602 citations

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Light-Scattering Submicroscopic Particles as Highly Fluorescent Analogs and Their Use as Tracer Labels in Clinical and Biological Applications

Juan Yguerabide, Evangelina E. Yguerabide · Analytical Biochemistry · 1998 · 540 citations

, , +12