Measurements of the true affinity constant in solution of antigen-antibody complexes by enzyme-linked immunosorbent assay

THE ATTRACTIONS OF PROTEINS FOR SMALL MOLECULES AND IONS

George Scatchard · Annals of the New York Academy of Sciences · 1949 · 19.9K citations

Natural Sciences, Biophysics, Chemistry +9

Isolation of pure IgG1, IgG2a and IgG2b immunoglobulins from mouse serum using protein A-Sepharose

Peter L. Ey, Stephen J. Prowse, C. R. Jenkin · Immunochemistry · 1978 · 2.7K citations

Life Sciences, Biochemistry, Biotechnology +13

The genetic control and cytoplasmic expression of “Inducibility” in the synthesis of β-galactosidase by E. coli

Arthur B. Pardee, François Jacob, Jacques Monod · Journal of Molecular Biology · 1959 · 1.1K citations

Life Sciences, Genetics, Molecular Biology +11

Synthesis and characterization of two fluorescent sulfhydryl reagents

Earl Nelson Hudson, Gregorio Weber · Biochemistry · 1973 · 427 citations

Natural Sciences, Biochemistry, Chemistry +5

A convenient enzyme-linked immunosorbent assay for testing whether monoclonal antibodies recognize the same antigenic site. Application to hybridomas specific for the β2-subunit of Escherichia coli tryptophan synthase

Bertrand Friguet, Lisa Djavadi‐Ohaniance, Jacqueline Pages et al. · Journal of Immunological Methods · 1983 · 244 citations

Life Sciences, Microbiology, Molecular Biology +16