Identification of novel molecular candidates for fatty liver in the hyperlipidemic mouse model, HcB19

Abstract

The inbred HcB19 mouse strain expresses a truncated form of thioredoxin interacting protein and is phenotypically characterized by fatty liver and elevated plasma triglycerides and VLDL. Recently, these mice have been proposed as an animal model for familial combined hyperlipidemia. The aim of the present study was identification of hepatic proteins specifically associated with the presence of fatty liver. Eighteen differential proteins were detected in whole-liver homogenate from HcB19, or the parental strain C3H, using 2D electrophoresis, and 11 of those were successfully identified by mass spectrometry. Five of the identified differential proteins were mitochondrial, two peroxisomal, two cytosolic, and two secretory. Four differential proteins were novel in the fatty liver proteome [i.e., aconitase, succinate dehydrogenase, propionyl CoA carboxylase α chain (PCCA), and 3-hydroxyanthranilate and of of in and have identified differential proteins in the hepatic proteome of mice with fatty and and differential of and in of fatty liver or in the of hyperlipidemia. The inbred HcB19 mouse strain expresses a truncated form of thioredoxin interacting protein and is phenotypically characterized by fatty liver and elevated plasma triglycerides and VLDL. Recently, these mice have been proposed as an animal model for familial combined hyperlipidemia. The aim of the present study was identification of hepatic proteins specifically associated with the presence of fatty liver. Eighteen differential proteins were detected in whole-liver homogenate from HcB19, or the parental strain C3H, using 2D electrophoresis, and 11 of those were successfully identified by mass spectrometry. Five of the identified differential proteins were mitochondrial, two peroxisomal, two cytosolic, and two secretory. Four differential proteins were novel in the fatty liver proteome [i.e., aconitase, succinate dehydrogenase, propionyl CoA carboxylase α chain (PCCA), and 3-hydroxyanthranilate and of of in and have identified differential proteins in the hepatic proteome of mice with fatty and and differential of and in of fatty liver or in the of hyperlipidemia. as hepatic or is characterized by the of the of in the of fatty liver. hepatic triglycerides of an of the liver in VLDL. been in with liver in familial is by the of hepatic in the liver an for and fatty liver associated with of in a of the from animal is fatty liver in with familial combined of fatty liver in familial combined as as and the in and in the of and fatty liver is a with the of liver or and for a study of from fatty liver by a in the of and and the or or of the a of and a of the from animal in these of in of these or a protein in of proteins as of protein of mice and mice with of the differential of and associated proteins in 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