Journal of Applied Physiology · 2000 · 699 citations · 27 references
EducationEndocrinologyPhysiologyExercise PhysiologyExercise TestingPhysical ActivityAerobic ExerciseExercise TherapyMetabolic Adaptation To ExerciseMetabolic Responses To ExercisePhysical FitnessResistance TrainingEndocrine Adaptation To ExerciseExercise-induced Growth Hormone SecretionHormonal Regulation Of Exercise MetabolismHormonal Response To ExerciseLow-intensity ExerciseResistance Exercise MetabolismTourniquet TechniqueVascular Occlusion Test
The study examined hormonal and inflammatory responses to low‑intensity resistance exercise with vascular occlusion. Six participants performed 14 repetitions × 5 sets of 20 % 1RM leg extensions while a thigh tourniquet applied ~214 mmHg, and plasma GH, NE, lactate, IL‑6, lipid peroxide, and CPK were measured before and after exercise. The occlusion protocol caused a transient, ~290‑fold increase in GH and marked rises in NE and lactate, while IL‑6 rose more slowly and remained slightly elevated 24 h later; lipid peroxide and CPK were unchanged, indicating that light resistance with occlusion stimulates GH via metabolite accumulation without tissue damage.
Hormonal and inflammatory responses to low-intensity resistance exercise with vascular occlusion were studied. Subjects ( n = 6) performed bilateral leg extension exercise in the seated position, with the proximal end of their thigh compressed at 214 ± 7.7 (SE) mmHg throughout the session of exercise by means of a pressure tourniquet. Mean intensity and quantity of the exercise were 20% of 1 repetition maximum and 14 repetitions × 5 sets, respectively. In each set, the subjects repeated the movement until exhaustion. Plasma concentrations of growth hormone (GH), norepinephrine (NE), lacate (La), lipid peroxide (LP), interleukin-6 (IL-6), and activity of creatine phosphokinase (CPK) were measured before and after the exercise was finished and the tourniquet was released. Concentrations of GH, NE, and La consistently showed marked, transient increases after the exercise with occlusion, whereas they did not change a great deal after the exercise without occlusion (control) done at the same intensity and quantity. Notably, concentration of GH reached a level ∼290 times as high as that of the resting level 15 min after the exercise. IL-6 concentration showed a much more gradual increase and was maintained at a slightly higher level than in the control even 24 h after exercise. Concentrations of LP and CPK showed no significant change. The results suggest that extremely light resistance exercise combined with occlusion greatly stimulates the secretion of GH through regional accumulation of metabolites without considerable tissue damage.
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A simple fluorometric assay for lipoperoxide in blood plasma
Kunio Yagi · Biochemical Medicine · 1976 · 2.1K citations
Evidence that interleukin‐6 is produced in human skeletal muscle during prolonged running
Thomas Rohde, M. Zacho, S. Asp et al. · The Journal of Physiology · 1998 · 672 citations · Full text