HPTLC evaluation of oleanolic acid and ursolic acid from the methanol extract of Wattakaka volubilis

Abstract

Objective: To find out the secondary metabolites present in the methanol extract of Wattakaka volubilis (W. volubilis). Methods: High performance thin layer chromatography method for the quantification of triterpenoids in soxhlet methanol extract of W. volubilis is described by densitometric scanning. The linear regression data from the calibration curve was plotted over the range of 10–25 μg/mL, r2 = 0.992 46, 0.950 42 respectively. A mixture of toluene: methanol (9:1) was used as mobile phase for oleanolic acid were petroleum ether: chloroform: ethyl acetate: methanol (4:1:0.1:0.1) were used for ursolic acid. Results: The results showed that the presence of oleanolic acid and ursolic acid in methanol extract. The content found to be 218.30 ng and 509.99 ng/10 mg of extract. Conclusion: For conclusion, above study scientifically validated as a useful traditional medicine with the identification of bioactive secondary metabolites.