CHROMATOGRAPHIC ASSAY OF MIXED PENICILLINS, AMPIGILLIN AND CLOXACILLIN IN BODY FLUIDS

Abstract

A thin-layer chromatographic assay method for the separation and determination of ampicillin and cloxacillin when present as a mixture was developed using a bioautographic technique. The measurement of the diameters of the inhibition zone was used as the means of quantitation. This method was useful for the separate determination of ampicillin and cloxacillin in the serum or urine from the animal or human subject administered with both penicillins.