Monitoring Molecular Recognition of the Ribosomal Decoding Site

Abstract

Adenine flips out: A combination of X-ray crystallography, 2-aminopurine fluorescence labeling, and the use of aminoglycosides as ligands is exploited to demonstrate conformational transitions in the RNA domain that ensures accurate protein synthesis (see picture). The triggering of a conformational change of an adenine unit in the RNA by ligand binding can be used as the basis of a screening method to discover antibiotics. Supporting information for this article is available on the WWW under http://www.wiley-vch.de/contents/jc_2002/2004/z54217_s.pdf or from the author. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.