Assessment of SCAR markers to design real‐time PCR primers for rhizosphere quantification of <i>Azospirillum brasilense</i> phytostimulatory inoculants of maize

Abstract

Effective root colonization is a prerequisite for successful Azospirillum phytostimulation, but cultivation-independent monitoring methods were lacking. The real-time PCR methods developed here will help understand the effect of environmental conditions on root colonization and phytostimulation by A. brasilense UAP-154 and CFN-535.