Fertility Restoration in Cytoplasmic-Nuclear Male-Sterile Lines Derived from 3 Wild Relatives of Pigeonpea

Abstract

Three cytoplasmic-nuclear male-sterile (CMS) lines, one each derived from Cajanus sericeus (A(1) cytoplasm), Cajanus scarabaeoides (A(2) cytoplasm), and Cajanus cajanifolius (A(4) cytoplasm), were crossed to 7 pigeonpea (Cajanus cajan (L.) Millsp.) cultivars in a line x tester mating scheme to study the fertility restoration of the CMS lines. Twenty-one F(1) hybrid combinations were planted in unreplicated 3-row plots in 3 environments. There was no effect of environments on the expression of fertility restoration. Pigeonpea cultivar ICPL 129-3 restored fertility in A(1) cytoplasm and maintained male sterility in the other 2 (A(2) and A(4)) cytoplasms. Among crosses involving CMS line (of A(4) cytoplasm) ICPA 2039 one hybrid combination was male-sterile and another male fertile. The remaining 5 combinations segregated for male-fertility (66-84% fertility restoration). Such testers can easily be purified for use in hybrid breeding programs by selfing and single-plant selection for 2-3 generations.