Protein and Peptide Letters · 2008 · 30 citations · 0 references
EngineeringBioengineeringBiotechnologyBiosensorsNanobiotechnologySensorsBiological SensorsDna BiosensorsDna NanotechnologyNanomaterial-based BiosensorsNanosensorsNucleic Acid BiosensorsAptamersArtificial ReceptorsMethod Detection LimitMulti-walled Carbon NanotubesProtein DetectionQuartz Crystal MicrobalanceQuartz Crystal Microbalance BiosensorsReceptors
We report on a new type of artificial receptor formed by hybridization of two DNA aptamers for human thrombin (aptabody). This aptasensor based on multiwalled carbon nanotubes allowed us to detect thrombin with detection limit of 0.3 nM, which was 3 times better in comparison with conventional aptamer. Keywords: DNA aptamers, Hybridization, Human thrombin, Multiwalled carbon nanotubes, Biosensor, Electrochemical quartz crystal microbalance