Molecular and Cellular Biology · 2002 · 77 citations · 45 references
Life SciencesDevelopmental BiologyGeneticsDevelopmental GeneticsEmbryogenesisGene ExpressionDevelopmental Reproductive PhysiologyMammalian DevelopmentSpermatogenesis GeneticsStress Response GenesStress-responsive Gene RegulationDna-binding MotifsEmbryonic Development GeneticsHeat Shock FactorsHeat Shock Protein RegulationHeat Shock ResponseHeat Shock Response GenesRegulation By Heat Shock ProteinsStress-inducible Gene Expression
Members of the heat shock factor (HSF) family are evolutionarily conserved regulators that share a highly homologous DNA-binding domain. In mammals, HSF1 is the main factor controlling the stress-inducible expression of Hsp genes while the functions of HSF2 and HSF4 are less clear. Based on its developmental profile of expression, it was hypothesized that HSF2 may play an essential role in brain and heart development, spermatogenesis, and erythroid differentiation. To directly assess this hypothesis and better understand the underlying mechanisms that require HSF2, we generated Hsf2 knockout mice. Here, we report that Hsf2(-/-) mice are viable and fertile and exhibit normal life span and behavioral functions. We conclude that HSF2, most probably because its physiological roles are integrated into a redundant network of gene regulation and function, is dispensable for normal development, fertility, and postnatal psychomotor function.
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Targeted disruption of the c-src proto-oncogene leads to osteopetrosis in mice
Philippe Soriano, Charles A. Montgomery, Robert S. Geske et al. · Cell · 1991 · 2K citations