Journal of General Virology · 1991 · 81 citations · 10 references
Veterinary MedicineInfectious DiseasesVeterinary MicrobiologyImmunology Of Infectious DiseasesMolecular VirologyVirologyAnimal VirusesImmunodeficiency DisordersLivestock Infectious DiseasesRuminant Infectious DiseasesVeterinary ImmunologyVeterinary VirologyBovine VirologyIndirect Immunofluorescence AssayLivestock Viral DiseasesLivestock VirologyOvine VirologyWestern Blot Analysis
Bovine immunodeficiency virus (BIV) was purified by isodensity centrifugation; viral activities were monitored in gradient fractions using the reverse transcriptase assay and a p26-specific monoclonal antibody ELISA. In the coincident peak fractions (density about 1.17 g/ml) proteins with Mr values of 26K, 17K, 53K, 14K and 100K (with decreasing intensity) were detected by Western blotting using serum of a calf after experimental BIV infection. When 957 randomly collected cattle sera from The Netherlands were tested by indirect immunofluorescence and confirmed using Western blot and/or radioimmunoprecipitation, 1.4% appeared seropositive. Thus BIV infection is not uncommon in one European cattle population.
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