Promicromonospora kroppenstedtii sp. nov., isolated from sandy soil

Abstract

A new actinobacterial strain, RS16(T), was isolated from sandy soil collected in Zamora, Spain, and was studied to determine its taxonomic position. A neighbour-joining tree based on 16S rRNA gene sequence analysis revealed that the novel isolate formed an independent branch between Promicromonospora sukumoe DSM 44121(T) and Promicromonospora citrea DSM 43110(T). Levels of 16S rRNA gene sequence similarity between the novel isolate and its phylogenetic neighbours ranged from 98.7 to 98.9 %. Chemotaxonomic properties, such as the predominant menaquinone and polar lipids, supported the assignment of the novel isolate to the genus Promicromonospora, however, a significant number of physiological differences were found between the novel isolate and the other recognized species of the genus Promicromonospora. On the basis of these results, it is proposed that strain RS16(T) represents a novel species of the genus Promicromonospora, for which the name Promicromonospora kroppenstedtii sp. nov. is proposed. The type strain is RS16(T) (=DSM 19349(T)=LMG 24382(T)).