Automatic annotation of organellar genomes with DOGMA

TLDR

DOGMA automates annotation of plant chloroplast and animal mitochondrial genomes. DOGMA is a web‑based tool that uses BLAST against a custom database and tRNA secondary‑structure conservation to annotate genes, offers a GUI for editing, stores annotations on a secure server, and exports results for GenBank submission. The tool and documentation are available at http://phylocluster.biosci.utexas.edu/dogma/.

Abstract

Abstract Summary: The Dual Organellar GenoMe Annotator (DOGMA) automates the annotation of organellar (plant chloroplast and animal mitochondrial) genomes. It is a Web-based package that allows the use of BLAST searches against a custom database, and conservation of basepairing in the secondary structure of animal mitochondrial tRNAs to identify and annotate genes. DOGMA provides a graphical user interface for viewing and editing annotations. Annotations are stored on our password-protected server to enable repeated sessions of working on the same genome. Finished annotations can be extracted for direct submission to GenBank. Availability: http://phylocluster.biosci.utexas.edu/dogma/ Supplementary information: Detailed documentation and tutorials for annotating both animal mitochondrial and plant chloroplast genomes can be found on the DOGMA home page.