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Glucose-6-phosphate Dehydrogenase Isoenzymes in Human Cell Cultures Determined by Sucrose-Agar Gel and Cellulose Acetate Zymograms
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1968
Year
Human Cell CulturesGeneticsCell CultureGlucose-6-phosphate Dehydrogenase IsoenzymesEnzymatic ModificationCellulose Acetate StripsSucrose-agar GelBioanalysisHematologyDonor RaceBiochemistryCell DivisionCell BiologyCellular EnzymologyNatural SciencesCellular BiochemistryMetabolismMedicineSummarycell Lines
SummaryCell lines and strains were studied with respect to possession of G-6-PD isoenzymes, using cellulose acetate strips and sucrose-agar gel electrophoretic media. The latter provided higher resolution of A and B bands. The A and B types of G-6-PD were found to occur in human, diploid cultured cell populations in accordance with racial origin of the donor; while certain other heteroploid cell lines were found to possess A type G-6-PD irrespective of donor race.