Characterization of Temperate Bacteriophages of <i>Bacillus subtilis</i> by the Restriction Endonuclease <i>Eco</i> RI: Evidence for Three Different Temperate Bacteriophages

Abstract

Temperate bacteriophages of Bacillus subtilis were characterized according to host range and digestion of the bacteriophage genome by endonuclease EcoRI. The three bacteriophages, phi3T, SPO2, and phi105, were all heteroimmune, and the DNA digests showed dissimilar patterns by agarose-ethidium bromide gel electrophoresis.