HPLC analysis of flavonoids in Eupatorium littorale

Brás Heleno de Oliveira, Tomoe Nakashima, José Dias de Souza Filho, Fabiano L. Frehse

Journal of the Brazilian Chemical Society · 2001 · 34 citations · 0 references

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Abstract

The isolation and identification of eupafolin and hispidulin in the dried leaves of Eupatorium littorale (Asteraceae) are described. A method for the detection of those flavonoids and rutin was also developed using reversed-phase HPLC with a photodiode array detector. Eupafolin and hispidulin were quantified and the concentrations found were 0.29% and 0.21%, respectively.