Analysis of the 5′ non‐coding region of rat liver <i>S</i>‐adenosylmethionine synthetase mRNA and comparison of the <i>M</i>_r deduced from the cDNA sequence and the purified enzyme

Abstract

A 3 kb cDNA coding for rat liver S-adenosylmethionine (AdoMet) synthetase has been isolated. The Mr of the protein has been unequivocally determined by cDNA sequencing and enzyme purification on a thiopropyl-Sepharose column. The length of the mRNA 5' non-coding region has been defined by primer-extension analysis. The rat liver cloned cDNA has been also used to detect S-adenosylmethionine synthetase mRNA in human liver.