A G‐box motif (GCCACGTGCC) tetramer confers high‐level constitutive expression in dicot and monocot plants

Abstract

Summary GUS reporter expression from 11 basal promoters (CaMV –90) with G‐box cores (CACGTG) was analysed to evaluate the regulatory roles of G‐box flanking sequences. While most G‐box motifs exhibited some tissue preference of gene expression, a distinct tissue‐specific expression was not apparent. However, one of 11 G‐box sequences, the G‐box 10 (GCCACGTGCC) tetramer, conferred a high‐level constitutive expression in seed, root, leaf, axillary bud, almost all parts of flower buds and pollen of transgenic tobacco plants. Furthermore, the G‐box 10 tetramer promoter exhibited high‐level expression in transgenic dicot carrot and monocot rice. This is apparently the first report of a G‐box motif conferring a high‐level constitutive expression in a non‐tissue‐specific manner.