PubMed · 1984 · 39 citations · 10 references
PharmacyMolecular BiologyToxicologyCarcinogenesisDna RepairMolecular ToxicologyDna DamageDna Double-strand Break RepairDna Repair In CarcinogenesisFish ToxicologyGenotoxic CarcinogenesisMutagenicity AssessmentDna Repair InductionDna Repair KineticsDna Repair MechanismsDna Repair PathwaysDna Repair Response AssessmentInduction In HepatocytesMammalian Cell Mutagenicity AssaysPrimary Cells
DNA repair synthesis was autoradiographically measured in liver, stomach, and intestinal cells isolated from rainbow trout which were exposed in vitro to the chemical mutagens, N-methyl-N'-nitro-N-nitrosoguanidine, 4-nitroquinoline 1-oxide, and aflatoxin B1. The level of repair was greatest in primary hepatocytes which responded to all three mutagens. Only nominal amounts of repair were detected in stomach cells following N-methyl-N'-nitro-N-nitrosoguanidine and 4-nitroquinoline 1-oxide exposures and in intestinal cells following 4-nitroquinoline 1-oxide exposure. In comparison with cultured rainbow trout cells, the quantity of DNA repair found in primary cells is significantly less.
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