Japanese Journal of Medical Science and Biology · 1978 · 23 citations · 12 references
Veterinary MedicinePoultry ScienceVeterinary MicrobiologyMolecular VirologyPoultry MedicineVirologyAnimal VirusesPoultry VirologyVeterinary VirologyViral Diseases Of PoultryViral InfectionsViral ZoonosesAntigenic TypingAvian Influenza In WildlifeAvian Influenza VirusAvian Influenza VirusesInfluenza ZoonosesViral Pathogenesis In WildlifeZoonotic Avian Viruses
Ortho- and parmyxoviruses were isolated at high frequencies from cloacal and tracheal samples of 334 domestic and 161 migrating ducks in Miyagi and Akita Prefectures, Japan, in the winter of 1976--77. Forty-four hemagglutinating agents were isolated from these ducks. The frequency of virus isolation was much higher from the cloacal samples (6.3%) than the tracheal samples (2.6%). However, no significant difference was found in the isolation ratio between the domestic (7.8%) and migrating (6.8%) ducks. Nine strains of the hemagglutinating agents were identified as influenza A viruses and confirmed to be in pure form by complement-fixation (CF) tests and from their polypeptide profiles. The antigenic characterization of the influenza A viruses revealed the presence of several subtypes; Hav5Nav-2-3, Hav6Neq2, Hav6N2, Hav7Neq2 and Hav7N2.
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Influenzavirus neuraminidase and neuraminidase-inhibition test procedures.
M Aymard-Henry, M. T. Coleman, W. R. Dowdle et al. · PubMed · 1973 · 421 citations