Autoradiographic studies of cerebellar histogenesis in the bullfrog tadpole during metamorphosis: The external granular layer

Abstract

Spontaneously metamorphosing bullfrog tadpoles and those induced to metamorphose by injections of thyroid stimulating hormone (TSH) were given a single intraperitoneal injection of thymidine-3H (10 muCi/g body weight). The brains were dissected out at various periods 3 hours to 14 days later, and processed for autoradiography. At the 3-hour interval after thymidine-3H injection, ependymal cells were labelled, but not the external granular layer (EGL) cells. Furthermore, in all the metamorphosing tadpoles intense labelling was restricted to the ependyma of the marginal region of the cerebellar plate. At 48 hours, labelled cells were seen in the EGL of the marginal region. At the 4-day interval, most of the EGL was labelled, and labelled cells were also seen migrating from the EGL into the internal granular layer (IGL). By 14 days, several labelled cells were seen in the IGL. Although the sequence of cerebellar histogenesis in the frog is similar to the general pattern described in other vertebrate groups, the results indicate that the EGL of the frog cerebellum does not serve the function of a secondary proliferating zone.