The Use of Quantitative Real-Time Reverse Transcriptase PCR for 5′ and 3′ Portions of <i>ALK</i> Transcripts to Detect <i>ALK</i> Rearrangements in Lung Cancers

Abstract

Real-time RT-PCR is a rapid and accurate method for diagnosing ALK-rearranged lung cancers. Coupling of 5' RACE to this method should further facilitate rapid identification of novel ALK fusion genes.