Simultaneous determination of salidroside, rosavin, and rosarin in extracts from Rhodiola rosea by high performance liquid chromatography with tandem mass spectrometry detection

Abstract

An approach is developed for the simultaneous determination of salidroside, rosarin, and rosavin in extracts from plant based on liquid chromatography/mass spectrometry; the detection limits of the method are 2, 4, and 6 ng/mL for salidroside, rosavin, and rosarin, respectively. The method was validated using real extracts from Rhodiola rosea rhizome. The analysis of extracts was accomplished by reversed-phase chromatography with a SB-C18 stationary phase. The analytes were detected by tandem mass spectrometry with electrospray ionization in the multiple reaction monitoring mode for negative ions.