Plant Regeneration from Cultured Immature Embryos and Inflorescences of 25 Cultivars of Wheat (<i>Triticum aestivum</i>)

Abstract

Immature embryos and inflorescences of wheat (&lt;it&gt;Triticum aestivum&lt;/it&gt;) were cultured on agar media. The presence of the auxin 2, 4-D induced the formation of multiple shoots in a proportion of cultures. In some cases the shoots developed from organized, embryoid-like structures. The morphogenetic capacities of 25 different cultivars of spring and winter wheat were compared, and clear differences were found between genotypes. Shoots were cultured separately, and the resulting plants grown to maturity in the glasshouse. Over a thousand plants were regenerated in this way, and phenotypic variation was observed amongst the regenerants.