Active Internalization of the <i>Penicillium chrysogenum</i> Antifungal Protein PAF in Sensitive Aspergilli

Abstract

The Penicillium chrysogenum antifungal protein PAF inhibits the growth of various filamentous fungi. In this study, PAF was found to localize to the cytoplasm of sensitive aspergilli by indirect immunofluorescence staining. The internalization process required active metabolism and ATP and was prevented by latrunculin B, suggesting an endocytotic mechanism.