Separation of NTO Related 1,2,4‐Triazole‐3‐One Derivatives by a high performance liquid chromatography and capillary electrophoresis

Abstract

Abstract This report describes a direct, rapid and sensitive method for separating and quantifying 5‐nitro‐1,2,4‐triazole‐3‐one 1 (NTO) and 5‐amino‐1,2,4‐triazole‐3‐one 2 . Analyses were performed on water and on soil containing compounds 1 and/or 2 , using reversed phase HPLC columns. A mixture of compounds 1 , 2 , Urazole 3 and 1,2,4‐triazole‐3‐one 4 was well separated by HPLC on a Hypercarb column packed with porous graphitic carbon, or by capillary electrophoresis. Both methods could be used to detect such compounds in the environment and monitor their biological and chemical degradation.