Agrobacterium-mediated transient expression system in banana immature fruits

Abstract

Transient expression assays are useful for saving time in studies of gene function, particularly ones expressed in fruit. Transient expression system of a reporter gene in immature fruit of banana was developed. Agrobacterium tumefaciens , whose plasmid contains an intron-containing Agrobacterium tumefaciens , whose plasmid contains an intron-containing Agrobacterium tumefaciens , whose plasmid contains an intron-containing
-glucuronidase ( gusA ) gene under regulatory control of the CaMV 35S promoter, was vacuum-infiltrated into sliced fruits and co-cultured. GUS histochemical assay was performed three days after co-cultivation, and a high level of GUS expression was observed. This transient expression system is useful for routine transient assays to validate genes expressed in banana fruit.