Expanding the Genetic Code for Photoclick Chemistry in <i>E. coli</i>, Mammalian Cells, and <i>A. thaliana</i>

Abstract

Breaking the (genetic) code: N-ε-acryl- lysine was genetically incorporated into proteins in bacterial cells, mammalian cells, and Arabidopsis thaliana by co-expressing a pyrrolysyl-tRNA mutant synthetase and its cognate tRNA in the model organism. A photoclick reaction driven by irradiation by 365 nm UV light or 405 nm laser light and allowed for site-specific protein labeling within minutes both in vitro and in vivo. As a service to our authors and readers, this journal provides supporting information supplied by the authors. Such materials are peer reviewed and may be re-organized for online delivery, but are not copy-edited or typeset. Technical support issues arising from supporting information (other than missing files) should be addressed to the authors. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.