Kinetic Determination of Serum Glucose by Use of the Hexokinase/Glucose-6-phosphate Dehydrogenase Method

Abstract

We have developed a kinetic fixed-time approach for the quantitative determination of serum glucose by use of the hexokinase/glucose-6-phosphate dehydrogenase method. To achieve a large measuring range, we have apparently increased the Michaelis constant of glucose-6-phosphate dehydrogenase through addition of the competitive inhibitor ATP. By this means, serum samples with glucose concentrations up to 55.5 mmol/l could be analyzed without pre-dilution. The method has been adapted to the ENI GEMSAEC analyzer and to the LKB 2086 Mark II analyzer. It yielded satisfactory results with regard to precision. A comparison of the kinetic procedure with the manual end-point method showed good agreement. No interferences from hemoglobin, bilirubin, or lipemia have been observed.