Real-Time PCR Assay Targets the 23S-5S Spacer for Direct Detection and Differentiation of <i>Legionella</i> spp. and <i>Legionella pneumophila</i>

Abstract

ABSTRACT A real-time PCR for the ABI Prism 7000 system targeting the 23S-5S spacer of Legionella spp. was developed. Simultaneous detection and differentiation of Legionella spp. and Legionella pneumophila within 90 min and without post-PCR melting-curve analysis was achieved using two TaqMan probes. In sputum samples from 23 controls and 17 patients with legionellosis, defined by positive culture, urinary antigen testing, or seroconversion, 94% sensitivity and 100% specificity were observed.