Carbohydrate‐Encapsulated Gold Nanoparticles for Rapid Target‐Protein Identification and Binding‐Epitope Mapping

Abstract

Carbohydrate–lectin recognition plays important roles in cell–cell communication, proliferation, and differentiation. We report here a new approach of using a carbohydrate-encapsulated gold nanoparticle (shown in purple) as an affinity probe for the efficient separation and enrichment of target proteins, and then protein identification and epitope mapping by MALDI-TOF MS. Supporting information for this article is available on the WWW under http://www.wiley-vch.de/contents/jc_2268/2005/z500023_s.pdf or from the author. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.